MEGAResFrom MEG, the Microbial Ecology Group

APH3-PRIME

Aminoglycoside O-phosphotransferases transfer a phosphate from ATP to a specific hydroxyl group on the aminoglycoside molecule. This confers resistance by drug modification. Aph(3') phosphotransferases modify the 3 prime hydroxyl group.

References

Other Groups in Aminoglycoside O-phosphotransferases

Sequences

Download Sequences

>AGly|AY339625.2|gene5|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACTTGGATGCTGATTTATATGGGTATAG
ATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCAATGCGCCAGAGTTGTTTC
TGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGCATTTATGCCT
CTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATT
CCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGA
TTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTG
GTTGATGCTAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCC
ATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTT
GTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCT
CCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCT
CGATGAGTTTTTCTGA

>CARD|phgb|BX664015|103017-103833|ARO:3002641|APH3-PRIME-Ia|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
TCAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCC
GTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACT
CGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGAC
TGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAA
AATCACTAGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGA
CAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATA
TTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAAT
GCTTGATGGTCGGAAGAGGCATAAATGCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTA
CCTTTGCCATGTTTCAGAAACAACTCTGGCGCATTGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGAC
ATTATCGCGAGCCCATCTATACCCATATAAATCAGCATCCAGGTTGGAATTTAATCGCGGCCTCGAGCAAGACGTTTCCC
GTTGAATATGGCTCATA

>627|X03364.1|X03364|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGAACGAAAGTACGCGTAATTGGCCGGAGGAACTTCTTGAGCTTCTCGGGCAGACGGAACTAACCGTCAACAAAATCGG
ATATTCCGGAGATCACGTCTATCACGTGAAAGAGTACAGGGGCACCCCCGCATTTCTGAAAATCGCCCCCAGTGTATGGT
GGAGAACGCTCCGGCCCGAAATTGAAGCGCTCGCTTGGCTGGACGGGAAGCTCCCGGTTCCCAAAATTTTGTACACGGCC
GAACACGGCGGGATGGACTACTTGCTGATGGAGGCGCTAGGCGGAAAAGACGGCTCCCACGAAACGATCCAGGCGAAGCG
GAAACTGTTTGTGAAGCTGTACGCGGAAGGGCTCCGAAGCGTGCATGGCCTCGATATCCGCGAATGTCCGCTGTCGAACG
GGCTGGAGAAGAAGCTCCGGGATGCGAAAAGAATAGTCGATGAGAGCCTGGTGGACCCGGCCGATATAAAAGAGGAGTAC
GATTGCACGCCGGAGGAATTGTACGGGCTATTGCTTGAGAGTAAGCCGGTAACCGAAGATCTGGTTTTTGCGCACGGAGA
TTACTGTGCTCCGAATCTGATTATCGACGGTGAGAAGCTGTCGGGATTTATCGATCTCGGACGTGCGGGCGTGGCGGACC
GTTATCAGGACATCAGCCTGGCGATCCGCAGCCTCCGGCACGATTACGGCGACGACCGCTACAAAGCGCTCTTCCTGGAA
CTTTACGGGCTGGACGGGCTTGACGAGGACAAGGTCCGGTATTACATCCGGCTGGATGAATTTTTTTGA

>AGly|aph3-prime-Ia_1_V00359|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAA
ATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTC
TGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCT
CTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATT
CCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGA
TTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTG
GTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCC
ATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTT
GTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCT
CCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCT
CGATGAGTTTTTCTAA

>AGly|aph3-prime-IIc_1_AM743169|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
TCACTTCTCCGCCAGCGCCTCCAGGGCCATGCGGGCGACTGTCTCGTCGATATCGGCCGGGTCGCCGTCATCCCTGAACC
ACACTGCCGACAGCGCAGTGCTGGCCGCGATCCAGCGCAGCAGGCGTGTGCGTTCCAGTTCGGACAGCGCACAGACCTGC
TCAAGGCGGATCGCAAACCGCTCGGGCCGCGTCGCAACGTGGATGCCCGGACCACAGAGGTCCGGGTTGCTGAACATCGT
CGTGTAATCGAACGCACGGTCCCCCAGCAGTCGCTTCGGGTCGATCGCCAACCAGCCGCGCGGGCCGAAATCCAGCACAT
TGTCATGATGCAGGTCGCCGTGCAGCGGGCGGATCTCCTGTTCGTCATGCAGCAGCCCCTCCGCCAGTGATCTGCACTGT
TCCAGCAGTGGCGGCAGGACAGCCCTTGGCTGCAGCAGGTCGGCGAACCATGTTCGCAGGCAGACCAGTTCCGCTGGCGG
CGCGCTCCGTGGCCGGTGCAGCCGCTGCAGGACCTGGCACAGGATCGCGGTGCAGGCGTCATCGTCGCCCTCGATCGAGC
GTTGCCGCAGCGAGTCACCGCGGGCACGCTCGATCAGGATCGCAGGCCCCTCGTGGGCCAGCAGGCGGGCGGCGCCATCG
CCGTTCCACCAGCGTAGCAGGCGATGACTGTTCTGCTCCTCGGTCTCGCTGCTGATCTTCAGCATCGCTGGCTCGCCCGC
AGCGGTCAGCACCGGCCAGAGCCGGGCGTGGGGCGTACGGAGGGCCGGGCCATCACGCCGCAATCGCCAGCGGCTCAGGT
AGGGTTCGCTCAT

>AGly|Aph3-VIb|AJ627643|4934-5713|780|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAATTGCCCAATATTATTCAACAATTTATTGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGA
TGTTTATTCTTTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTG
TCTCTCGTGAAGCGAAAATGTTGAGTTGGCTCTCTGATAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAG
CAGTTTGAATTCATGATCACTAAAGCGATCAATGCAAAATCAATTTCAGCGCTTTTTTTAACAGAGCAAGAATTGCTTGC
TATCTATAAGGAAACACTCAATCAGTTAAATGCAGTTGCTATTATTGATTGCCCATTTATTTCAAGCATTGATCATCGGT
TAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGAGATAGATCAAGATGATTTTGAGGCTGAATTATGGGGA
GACCATAAAACTTACATAAGTCTTTGGAATGAGTTAAATGAGACTCGTGTTGAAGAAAGATTGGTTTTTTCTCATGGCGA
TATCACGGATAGTAATATTTTTATAGATAAATCTGGTGAAATTTACTTTTTAGATCTTGGTCGTGCTGGATTAGCAGATG
AATTTGTAGATATATCTTTTGTTGAACGTTGCCTAAGAGAGGATGTATCTGAGGAAACTGCTAAAATATTTTTAAAGCAT
TTAAAAAACGATATGCCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA

>AGly|Aph3-Vb|M22126|373-1164|792|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAAAGCACGTTGCGCCGGACATACCCGCACCACACTTGGCACCTCGTGAACGAAGGAGACTCGGGCGCCTTCGTCTA
CCGCCTCACCGGACACGGGCCCGAGCTCTACGCGAAGATCGCCCCCCGCACCCCCGAGAACTCCGCCTTCCACCTCGACG
GCGAGGCCGACCGCCTCGACTGGCTCGCCCGCCATGGCATCTCGGTCCCCCGTGTCGTCGAGCGCGGTGCCGACGACACC
ACCGCCTGGCTCGTCACCGAGGCCGTGCCCGGCGCCGCGGCCTCCGAGGAGTGGCCCGAGGACGAGCGGGCGGCCGTTGT
CGACGCGATCGCCGAAATGGCCCGCACCCTCCATGAACTCCCCGTGTCCGAGTGCCCCTTCGACCGCCGCCTCGACGTCA
CCGGCGAGGCCCGGCACAACGTCCGCGAGGGCCTGGTCGACCTCGACGACCTCCAGGAGGAGCCGGCCGGCTGGACCGGC
GACCAACTCCTGGCCGAACTCGACCTGACGCGGCCCGAGAAGGAGGACTTGGTCGTCTGCCATGGCGACCTGTGCCCCAA
CAACGTGCTGCTCGACCCCGAGACCCACCGGATCACCGGGCTGATCGACGTCGGCCGCCTCCGGCTCGCCACCTGCCACG
CCGACCTCGCCCTCGCCGCCCGCGAACTGGCGATCGACGAGGACCCGTGGTTCGGCCCCGCATACGCCGAACGGTTCCTC
GAACGGTACGGGGCCCACCACGTCGACCAGGAGAAGATGGCCTTCTACCAGCTGCTCGACGAGTTCTTCTAG

>AGly|AphVIII|AF182845|1-804|804|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGACGATGCGTTGCGTGCACTGCGGGGTCGGTATCCCGGTTGTGAGTGGGTTGTTGTGGAGGATGGGGCCTCGGGGGC
TGGTGTTTATCGGCTTCGGGGTGGTGGGCGGGAGTTGTTTGTCAAGGTGGCAGCTCTGGGGGCCGGGGTGGGCTTGTTGG
GTGAGGCTGAGCGGCTGGTGTGGTTGGCGGAGGTGGGGATTCCCGTACCTCGTGTTGTGGAGGGTGGTGGGGACGAGAGG
GTCGCCTGGTTGGTCACCGAAGCGGTTCCGGGGCGTCCGGCCAGTGCGCGGTGGCCGCGGGAGCAGCGGCTGGACGTGGC
GGTGGCGCTCGCGGGGCTCGCTCGTTCGCTGCACGCGCTGGACTGGGAGCGGTGTCCGTTCGATCGCAGTCTCGCGGTGA
CGGTGCCGCAGGCGGCCCGTGCTGTCGCTGAAGGGAGCGTCGACTTGGAGGATCTGGACGAGGAGCGGAAGGGGTGGTCG
GGGGAGCGGCTTCTCGCCGAGCTGGAGCGGACTCGGCCTGCGGACGAGGATCTGGCGGTTTGCCACGGTGACCTGTGCCC
GGACAACGTGCTGCTCGACCCTCGTACCTGCGAGGTGACCGGGCTGATCGACGTGGGGCGGGTCGGCCGTGCGGACCGGC
ACTCCGATCTCGCGCTGGTGCTGCGCGAGCTGGCCCACGAGGAGGACCCGTGGTTCGGGCCGGAGTGTTCCGCGGCGTTC
CTGCGGGAGTACGGGCGCGGGTGGGATGGGGCGGTATCGGAGGAAAAGCTGGCGTTTTACCGGCTGTTGGACGAGTTCTT
CTGA

>237|S81599.1|S81599|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGTACGCCATGTTGCGCCGGAAATACCAGCACTACGAATGGACCTCCGTGAACGAAGGAGATTCGGGCGCCTCCGTTTA
CCGCCTCGCCGGACAGCAGCCCGAGCTCTATGTGAAATTCGCTCCGCGCGAACCGGAAAATTCCGCGTTCGACCTGGCGG
GCGAGGCCGACCGGCTCACCTGGCTCACCCGCCACGGCATCCCGGTTCCGTGCATTGTCGAGTGCGGCGGCGACGACACC
TCGGTTTTCCTCGTCACCGAGGCCGTCACCGGCGTATCGGCCGCCGAGGAGTGGCCGGAGCACCAGCGCTTCGCCGTCGT
CGAGGCGATGGCCGACCTCGCCCGCACCCTGCACGAACTGCCCGTTGGTGGCTGCCCCTTCGATCGCAGCCTGGCGGTGA
CGGTTGCCGAAGCCCGCCACAACCTACGCGAGGGCCTCGTGGACCTGGACGACCTCCAAGAGGAGCACGCCAACTGGTCC
GGTGACCAGCTTCTCGCCGAGCTCGACCGAACGCGGCCCGAGAAAGAGGATCTGGTCCTCTGCCACGGGGACCTGTGCCC
CAACAACGTGCTGCTCGATCCCGAGACATGCCGAGTCACCGGAATGATCGATGTGGGCCGCCTCGGCCGCGCCGATCGCC
ACGCCGACCTGGCCCTCGCCGCCCGCGAGCTGGAGATCGACGAGGATCCCTGGTTTGGCCCCGAGTACGCCCAGCGGTTC
CTCGAACGCTACGGCGCGCACCACGTCGACGAGAACAAGATGGCCTTTTACCAGCTGCTCGACGAGTTTTTCTAG

>578|X90856.1|X90856|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGCATGATGCAGCCACCTCCATGCCGCCGCAGGCTCCCTCAACCTGGGCCGACTACCTTGCCGGCTACCGCTGGCGAGG
GCAGGGCGAAGGATGTTCCGCGGCCACGGTCCACCGCCTGGAGGCTGCGCGGCGGCCGACCCTGTTCGTCAAGCAGGAAG
TGCTGTCCGCACATGCCGAGCTGCCCGCCGAAATCGCCCGCCTGCGCTGGCTGCACGGTGCCGGCATCGACTGCCCGCAG
GTGCTGAACGAAACCCAGAGCGACGGCCGGCAATGGCTGCTGATGAGCGCAATGCCGGGGGACACGCTGTCCGCGCTGGC
GCAGCGCGACGAGCTGGAGCCCGAGCGCCTGGTGCGCCTGGTGGCCGCCGCCCTGCGCCGGCTGCACGATCTCGATCCGG
CCGCCTGTCCCTTCGATCATCGCCTGGAACGGCGTCTGGACACCGTGCGCCAGCGGGTCGAGGCCGGGCTGGTGGACGAG
GCGGACTTCGACGACGACCATCGCGGTCGCAGCGCCACGGAGCTGTACCGCCTGCTGCTCGACCGGCGTCCGGCGGTCGA
AGACCTGGTGGTCGCCCACGGCGACGCCTGCCTGCCCAACCTCTTGGCGGAGGGCCGGCGCTTCAGCGGCTTCATCGATT
GCGGGCGGCTCGGCGTCGCCGACCGGCACCAGGACCTGGCCCTGGCCGCGCGGGACATCGAGGCCGAACTCGGCGCGGCC
TGGGCCGAGGCCTTCCTCGTCGAATACGGCGGCGATATCGACGGCGAACGGCTGGCGTACTTCAGGCTATTGGACGAGTT
CTTCTAG

>CARD|phgb|470266717|8362-9142|ARO:3003687|APH3-PRIME-VI|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAATTGCCCAATATTATTCAACAATTTATTGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGA
TGTTTATTCTTTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTG
TCTCTCGCGAAGCGAAAATGTTGAGTTGGCTCTCTGAGAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAG
CAGTTTGAATTAATGATCACTAAAGCGATCAATGCAAAACCAATTTCAGCGCTTTTTTTAACAGACCAAGAATTGCTTGC
TATCTATAAGGAGGCACTCAATCTGTTAAATTCAGTTGCTATTATTGATTGTCCATTTATTTCAAACATTGATCATCGGT
TAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGATATAGATCAAGATGATTTTGACGCTGAATTATGGGGA
GACCATAGAACTTACCTAAGTCTATGGAATGAGTTAACTGAGACTCGTGTTGAAGAAAGATTGGTTTTTTCTCATGGCGA
TATCACGGATAGTAATATTTTTATAGATAAATTCAATGAAATTTACTTTTTAGATCTTGGCCGTGCTGGGTTAGCTGATG
AATTTGTAGATATATCCTTTGTTGAACGTTGCCTAAGAGAGGATGCCTCGGAGGAAACTGCTAAAATATTTTTAAAGCAT
TTAAAAAATGATAGACCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA

>AGly|aph3-prime-Va_1_K00432|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGACGACAGCACGTTGCGCCGGAAGTACCCGCACCACGAGTGGCACGCAGTGAACGAAGGAGACTCGGGCGCCTTCGT
CTACCAGCTCACCGGCGGCCCCGAGCCCCAGCCCGAGCTCTACGCGAAGATCGCCCCCCGCGCCCCCGAGAACTCCGCCT
TCGACCTGTCCGGCGAGGCCGACCGGCTGGAGTGGCTCCACCGCCACGGGATCCCCGTCCCCCGCGTCGTCGAGCGCGGT
GCCGACGACACCGCCGCGTGGCTCGTCACGGAGGCCGTCCCCGGCGTCGCGGCGGCCGAGGAGTGGCCCGAGCACCAGCG
GTTCGCCGTGGTCGAGGCGATGGCGGAGCTGGCCCGCGCCCTCCACGAGCTGCCCGTGGAGGACTGCCCCTCCGACCGGC
GCCTCGACGCGGCGGTCGCCGAGGCCCGGCGGAACGTCGCCGAGGGCTTGGTGGACCTCGACGACCTGCAGGAGGAGCGG
GCCGGGTGGACCGGCGACCAGCTCCTGGCGGAGCTCGACCGCACCCGTCCCGAGAAGGAGGACCTGGTCGTCTGCCATGG
CGACCTGTGCCCCAACAACGTCCTGCTCGACCCCGGGACCTGCCGGGTCACCGGCGTGATCGACGTCGGCCGCCTCGGGG
TCGCCGACCGCCACGCCGACATCGCCTTGGCCGCCCGCGAGCTGGAGATCGACGAGGACCCCTGGTTCGGCCCCGCCTAC
GCCGAGCGGTTCCTGGAGCGGTACGGCGCCCACCGCGTCGACAAGGAGAAGCTGGCCTTCTACCAGCTTCTCGACGAGTT
CTTCTAG

>592|HQ424460.1|HQ424460|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAAGCTTCCAATCCCTTCACTGATGGCCTGCGGCTGCCGCGCGCATGGCAGGAAGCGTTGGCCGATGCGCACATCGA
GCGGCAGTCGATCGGCGTGTCGCGCGCGGATGTCGCGCGGGTGCATCGTCCCGGGCAGACCGACGCCTTCCTGAAATCGG
AAGTGATCGATGCCTTCAGTGAACTGGGTGATGAGATCGCCCGGCTGCGTTGGCTGCAGGCGCAGGGGCAGTCGGCGCCG
ACGGTGATTGCCACGACCGAGGAGGGCGGTCGGCGCTGGTTGTTGATGAGCGCGTTGCCCGGCCGCGACTTGGCCTCCTC
GCCGGAGCTCGCGCCGAGACGGGTGGCAGAACTGCTGGCTGACGCACTGCGTGGCCTGCATGCCGTGCCTGTAGCCAACT
GCCCGTTCGACCAGCAGTTGGCATCGCGCCTGCAGGCCGCACAGGCACGCGTCGAGGCGGGGCTGGTCGATGCCGATGAC
TTCGACGACGAGCGGCTGGGCCAGAGCCCGCAGCAGGTTTTCGCCGAGCTGCGCGCTACCCGGCCCGCTCATGAAGACCT
GGTGGTCAGTCAGGGCGATGCCTGCCTGCCCAACCTGACGGTGACCGATGGGCGGTTCACTGGCTTCATCGATTGTGGCC
GGTTGGGCGTGGCCGACCGCTATCAGGACCTGGCCCTGGCCGCGCGCAGCCTGGTCCACAATTTCGGGGAGAGCCGCTGT
GTCGCCGCGCTGTTCCAGCGCTACGGTGCGGTCCCTGATGAGCGGCGGCTTGCATTCTATCGGTTGCTTGACGAGTTTTT
CTGA

>611|Y18050.2|Y18050|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGACAGTCGCCCTCGACGAAGTATCTGAACTAAAGAATTTGCTTTCACCCTTGTTGGATGAATGCACTTTTGAAGAAGT
TGAGTATGGTCAGTCAGATGCTCGAGTGATTCGAGTTCTATTTCCTGATCGCAATACCGCGTATCTAAAGTACGCCTCCG
GATCTTCTGCTCAAGAAATTCTTCAAGAGCATCAGCGCACTAGATGGCTCAGAACACGAGCTCTCGTACCGGAAGTGATC
TCATATGTCTCGACTTCAACTGTCACCATCCTGTTGACAAAAGCATTGATTGGCCACAATGCCGCTGACGCCGCAGATGC
AGATCCAGTTATTGTTGTTGCAGAGATGGCACGAGCGTTACGCGACCTCCATTCGATCTCGCCTGACGATTGCCCATTCG
ACGAAAGGCTCCACCTGCGACTGAAGCTGGCTTCGGGCCGTTTGGAAGCCGGGTTAGTTGATGAGGAGGACTTTGATCAC
GCAAGGCAAGGCATGCTGGCGCGGGATGTTTACGAGCAACTTTTTATACAAATGCCTGGAGCGGAGCAGCTGGTAGTCAC
ACATGGCGACGCCTGTCCCGAGAACTTCATCTTCCAAGGTAATGCCTTCGTCGGCTTCATAGACTGCGGTCGGGTCGGGC
TTGCCGATAAGTATCAAGACCTGGCGCTTGCATCGAGAAACATTGACGCGGTATTTGGACCAGAACTCACTAACCAGTTC
TTCATCGAGTATGGAGAGCCAAATCCGAACATAGCTAAGATTGAGTACTACCGGATCTTGGATGAGTTCTTCTAA

>576|M20305.1|RP43APHA|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
GTGAACGATATTGATCGAGAAGAGCCCTGCGCAGCCGCTGCCGTGCCCGAGAGCATGGCGGCTCACGTGATGGGATACAA
ATGGGCGCGTGATAAGGTTGGTCAGTCCGGCTGCGCGGTCTATCGGCTGCATAGCAAGTCAGGCGGCTCCGACTTGTTTC
TGAAGCACGGCAAAGATGCTTTTGCCGACGACGTGACTGATGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCT
GTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATA
TCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGA
TCCCAGTGAGCGAATGCTCCGTTCAACAGTGGACCACGCATGCAGGCTTGCCCGAGCGCGGGAGTATCGAGGCGGGGGTT
GTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACC
GCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCT
GCATCGACGTTGGGCGGGCTGGTATTGCTGATCGATACCAAGACCTTGCCGTGTTATGGAACTGTCTTGAGGAGTTCGAA
CCTTCGCTTCAGGAGAGGCTTGTTGCGCAATATGGCATTGCCGATCCGGATAGGCGCAAGCTGCAATTTCATCTCCTGCT
GGACGAACTTTTCTAA

>AGly|AphA6|JF949760|695-1474|771|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAATTGCCCAATATTATTCAACAATTTATCGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGA
TGTTTATTCTTTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTG
TCTCTCGTGAAGCGAAAATGTTGAGTTGGCTCTCTGAGAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAG
CAGTTTGAATTCATGATCACTAAAGCGATCAATGCAAAACCAATTTCAGCGCTTTTTTTAACAGACCAAGAATTGCTTGC
TATCTATAAGGAGGCACTCAATCTGTTAAATTCAATTGCTATTATTGATTGTCCATTTATTTCAAACATTGATCATCGGT
TAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGATATAGATCAAGATGATTTGGACACTGAATTATGGGGA
GACCATAAAACTTACCTAAGTCTATGGAATGAGTTAACCGAGACTCGTGTTGAAGAAAGATTGGTTTTTTCTCATGGCGA
TATCACGGATAGTAATATTTTTATAGATAAATTCAATGAAATTTATTTTTTAGATCTTGGTCGTGCTGGGTTAGCAGATG
AATTTGTAGATATATCCTTTGTTGAACGTTGCCTAAGAGAGGATGCATCGGAGGAAACTGCGAAAATATTTTTAAAGCAT
TTAAAAAATGATAGACCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA

>AGly|Aph3_prime-VIa_1_X07753|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGAATTGCCCAATATTATTCAACAATTTATCGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGA
TGTTTATTCTTTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTG
TCTCTCGTGAAGCGAAAATGTTGAGTTGGCTCTCTGAGAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAG
CAGTTTGAATTCATGATCACTAAAGCGATCAATGCAAAACCAATTTCAGCGCTTTTTTTAACAGACCAAGAATTGCTTGC
TATCTATAAGGAGGCACTCAATCTGTTAAATTCAATTGCTATTATTGATTGTCCATTTATTTCAAACATTGATCATCGGT
TAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGATATAGATCAAGATGATTTTGACACTGAATTATGGGGA
GACCATAAAACTTACCTAAGTCTATGGAATGAGTTAACCGAGACTCGTGTTGAAGAAAGATTGGTTTTTTCTCATGGCGA
TATCACGGATAGTAATATTTTTATAGATAAATTCAATGAAATTTATTTTTTAGATCTTGGTCGTGCTGGGTTAGCAGATG
AATTTGTAGATATATCCTTTGTTGAACGTTGCCTAAGAGAGGATGCATCGGAGGAAACTGCGAAAATATTTTTAAAGCAT
TTAAAAAATGATAGACCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA

>700|M29953.1|CAJAPHA7A|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGAAATATATCGATGAAATTCAAATTCTGGGAAAATGTTCAGAGGGTATGTCTCCAGCAGAAGTATATAAATGCCAGCT
TAAAAATACTGTATGCTATCTGAAAAAAATTGACGATATATTTTCAAAAACCACATACAGCGTGAAAAGAGAAGCTGAGA
TGATGATGTGGTTATCCGATAAACTGAAAGTACCAGATGTAATCGAATACGGAGTACGAGAACATTCAGAATATTTGATC
ATGAGTGAGTTAAGGGGGAAACACATAGATTGCTTTATTGATCATCCAATAAAATATATTGAGTGCTTGGTAAACGCACT
TCATCAGCTACAAGCAATAGATATAAGAAACTGCCCATTTTCATCCAAAATAGATGTTCGATTAAAAGAACTAAAATATC
TTTTGGATAACAGAATTGCCGATATTGATGTATCGAATTGGGAAGATACAACAGAATTTGATGATCCAATGACGTTATAT
CAGTGGCTTTGCGAAAATCAACCTCAAGAAGAACTGTGTCTCTCTCATGGAGATATGAGCGCTAATTTTTTTGTATCTCA
TGATGGAATATATTTTTATGATTTGGCAAGATGTGGAGTTGCAGACAAATGGTTGGATATAGCATTTTGTGTCAGAGAGA
TTCGAGAATATTATCCTGATTCTGATTATGAAAAATTCTTTTTTAACATGTTGGGACTTGAACCGGATTATAAAAAAATT
AACTATTACATTTTATTAGATGAGATGTTTTAG

>AGly|Aph3-Va|U24442|680-1456|780|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGTCACCACCATCCCCGGTGCTGAAGAATTTCGAAGCATGGACGATGCGTTGCGTGCACTGCGGGGTCGGTATCCCGGT
TGTGAGTGGGTTGTTGTGGAGGATGGGGCCTCGGGGGCTGGTGTTTATCGGCTTCGGGGTGGTGGGCGGGAGTTGTTTGT
CAAGGTGGCAGCTCTGGGGGCCGGGGTGGGCTTGGGTGAGGCTGAGCGGCTGGTGTGGTTGGCGGAGGTGGGGATTCCCG
TACCTCGTGTTGTGGAGGGTGGTGGGGACGAGAGGGTCGCCTGGTTGGTCACCGAAGCGGTTCCGGGGCGTCCGGCCAGT
GCGCGGTGGCCGCGGGAGCAGCGGCTGGACGTGGCGGTGGCGCTCGCGGGGATCGCTCGTTCGCTGCACGGCGCTGGACT
GGGAGCGGTGTCCGTTCGATCGCAGTCTCGCGGTGACGGTGCCGCAGGCCCGTGCTGTCGCTGAAGGGAGCGTCGACTTG
GAGGATCTGACGAGGAGCGGAAGGGGTGGTCGGGGGAGCGGCTTCTCGCCGAGCTGGAGCGGACTCGGCCTGCGGACGAG
GATCTCGGTTTGCCACGGTGACCTGTGCCCGGACAACGTGCTGCTCGACCCTCGTACCTGCGAGGTGACCGGGCTGATCG
ACTGGGGCGGGTCGGCCGTGCGGACCGGCACTCCGATCTCGCGCGGTGCTGGCGTCGCGCGAGCTGCGCGGGTGGGTCGG
GGGCGGTGCAATCGGAGGAAAAGCTGCGTTTACCGGCTGTTGGACGAGTTCTTCTGA

>AGly|Aph3-Ia|HQ840942|23569-24384|816|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAA
ATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTC
TGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAAATGGTCAGACTAAACTGGCTGACGGAATTTATGCCT
CTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATT
CCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGA
TTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTG
GTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCC
ATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTT
GTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCT
CCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCT
CGATGAGTTTTTCTAA

>AGly|APH-Stph|HE579073|1778413-1779213|801|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
GTGGACGAGTTGGAGCAGTTTTATCAATTAGGGTGGACACTTGATTCAGCAGGTGGTGCATCTGGTGAAGCATATATGGC
TGAACAAGATGGACAAAAGTTGTTTTTAAAACGAAATTCAAATCCATTTATTGCGGCATTATCAGCAGAAGGTATTGTGC
CCAAATTAGTATGGACGAAACGCATAGAAACAGGCGAGGTTGTTACAGCACAACATTGGAAAAATGGGCGTGAACTATCT
TCAAACGAAATGAAGCAAACAAGAGTTGCACATTTATTAAAGAAGATACACAATTCTAGACCTTTATTAAGTATGTTAAA
GCGTATGGAAATGGAACCTATTACTCCTGAGATTATGCTTAATAAAATTAATGCCTCTTTATCAAGAGAAGTTTTAACAC
ATCATATTGTGAGAAAATCATTAACCTATTTAGAAGAGCATATACCGAGTTTAGATTCGCGTTTTTTCACTGTTGTACAT
GGTGATGTGAATCATAATAATTGGTTATTATCCGATCGAGATGAACTATTTTTAGTTGATTGGGAAGGTGCAATGATTGC
AGACCCGGCAATTGATATCGGAATGCTGCTTTACAACTATGTTCCACAACAACAATGGTCTGAATGGCTTGAAACATATG
GTGTTCAAGAGAGCTTGAATTTAAATAAACGTATGAAATGGTATACGGTAATTCAATCTATAGGTCTTGTTCAATGGTAT
GAAGAGCAAAAAAGATATAAGGATATGAACACTTGGCTTAAATTCCTAAATGAAGTTATGAATAGTAACATGTTTATTTA
A

>605|V01547.1|V01547|Aminoglycosides|Aminoglycoside_O-phosphotransferases|APH3-PRIME
ATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCGCTGCGTAAAAGATACGGAAGGAATGTC
TCCTGCTAAGGTATATAAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAAGGGACCA
CCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAA
CGGCATGATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAGTATGAAGATGAACAAAG
CCCTGAAAAGATTATCGAGCTGTATGCGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGA
ATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTGGCCGATGTGGATTGCGAAAACTGGGAA
GAAGACACTCCATTTAAAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTGTCTTTTC
CCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCA
GGGCGGACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGATCAGGGAGGATATCGGGGAAGAACAGTATGTCGAGCTA
TTTTTTGACTTACTGGGGATCAAGCCTGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAG